Chapter
6:
- Compare and contrast facilitated diffusion to active transport by an ABC transporter.
Chapter
7:
- Explain the process of binary fission. Include information about the DNA replication and the separation of the daughter cells (cytokinesis). The answer should include the following terms: MreB. ori, ter, ftsZ ring, formation of the septum.
- You have counted a biological sample using both a direct count method and a viable count method. The numbers are not the same; in fact the direct cell count is higher. Give at least two explanations for how this can be true. If you tell me it was a human error, you will need to be quite specific what the error was that caused the problem. Although making an error in the dilution series is a true reason, it is not an acceptable answer.
- What is a biofilm and why is it said to be heterogenic in nature? Why is it so hard to treat a biofilm with antimicrobial agents? (Give at least 2 reasons)
Chapter
8:
- What is the purpose of a biological safety cabinet? Also describe the operation and components of a biological safety cabinet.
- Briefly explain how the effectiveness of antimicrobial agents varies with population size, population composition, concentration or intensity of the agent, contact time, temperature, and local environmental conditions.
Chapter
9:
1. Consider
the following diagram of the energy flow for a particular reaction.
Is the reaction exergonic or endergonic? What does the diagram
indicate? How would the use of an enzyme catalyst affect the energy
flow? Indicate this on the diagram, and also indicate the energy of
activation and the free energy change of both the catalyzed and
uncatalyzed reactions.
A
+ B C + D
Time
Chapter
10:
1.
What is the function of the electron transport chain? How does it
relate to proton motive force, and what are the possible
roles/outcomes of the proton motive force in microorganisms?
2.
The authors make the statement,
“The use of a few common catabolic pathways, each degrading many
nutrients, greatly increases metabolic efficiency by avoiding the
need for a large number of less metabolically flexible pathways.”
Explain the significance of this statement.
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